I. Introduction II. DNA
 A. DNA and Chromosomes
B. DNA composition
C. From DNA to protein III. Gene Variation
A. Selective breeding -- using
 variations of nature
B. Mutations -- creating new
 variations
C Modern applications IV. Genetic Engineering
A. Overview of the basic steps in
 genetic engineering
B. Diabetes and the need for insulin
C. A method for producing human
 insulin in bacteria
 1. Isolating and cutting DNA
 2. Cloning DNA
 3. Vectors
 4. Making recombinant DNA
 5. Transformation
 6. Probing the gene library to identify
  colonies with the desired gene -
  DNA & RNA probes
 7. Mass production of the gene
  product
 8. Expressing eukaryotic genes |
V. Examples of modern genetic
 engineering
A. Genetic engineering in microbes
 1. Human growth hormone
  production in bacteria
 2. Bovine growth hormone
 3. Waterproof glue
 4. Cheese from microbes
B. Genetic engineering in plants
 1. Methods for transferring
  DNA through the cell wall
 2. Bacillus thuringiensis
  insecticide
 3. Longer lasting tomatoes
 4. Frost protection in strawberry
  plants
 5. Nitrogen fixation
C. Genetic engineering in animals
 1. Animals that produce
  products for humans
 2. Animal cloning
D. Genetic engineering in health
 care
 1. Traditional vaccines
 2. Genetically engineered
  vaccines
E. Genetic engineering in humans
 1. Gene therapy VI. Conclusion
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